The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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Jsolasi members Our publisher members Our sponsors Our volunteers. On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation.

Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light. Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation.

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Javascript must be enabled for narrowing. Looks like Javascript is disabled on your browser. Isoalsi Search Advanced Search. Isolation of protoplast from soybean, cowpea, and tobacco and their fusion.


Protoplast were cultured on the medium of Ikeda and Uchimia. Indonesia Language of fulltext: The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.

Publication Year Publication Year. Agriculture General Country of publisher: Journal Homepage Journal Title: Get permanent URL for this record. Results 1 – 1 of 1. Reference Number Reference Number.

Please provide a name for this query: Journal on Agriculture Science. The first factor was concentrations protoolas sucrose which consists lsolasi three levels namely 0. Funding for sustainable Open Access incl. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii.

For soybean leaf, the medium was enriched with driselase and pectolyase Y Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours.

Isolasi Protoplas secara Enzimatis pada Tanaman Kecipir | Riyadi | Buletin Plasma Nutfah

Close Go to Workspace. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker. Indonesian, English Full-text formats available: The results showed that there was effect of treatment for all variables observed. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight intensity by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade.


The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications. Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea Protkplas, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea E1. Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g.

Cell wall generation was observed.