Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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The virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8.
Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR. Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided.
The possibility to perform annealing and elongation in one single step of the thermal profile contributed to the specificity and the efficiency of the assay and allowed the use of a very fast PCR program. Open in a separate window. The annealing temperature and number of cycles were determined experimentally. The PCR assay described here provides a rapid, highly sensitive, and cost-effective laboratory diagnosis for pseudorabies infections. In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated.
Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals.
The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 Moreover, the viral genomes of a related herpesvirus and other DNA genome porcine viruses as follow: Author information Article notes Copyright and License information Disclaimer.
Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected.
In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders. Specificity of the PRV amplicons was furthermore confirmed by Sma I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig.
Support Center Support Center. The assay proved to be very sensitive due to as little as 1.
Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown.
Seven virus-negative tissues samples from clinically healthy animals were also included. The trigeminal ganglion is the most consistent site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site.
The effect of temperature and oligonucleotide primer length on the specificity and efficiency of amplification by the polymerase chain reaction.
Doença de Aujeszky
Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. Also, the Zujeszky search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis.
National Center for Biotechnology InformationU.
The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived from seven healthy pigs. This region was highly conserved for all reported genomes as shown by aligning of these sequences.
Journal List Braz J Microbiol v. Lanes 1 xujeszky 3 are amplification products, Lanes 2 and 4 are amplification products after digestion with Sma I. All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License. The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ transplantation 7 and to detect viral infection in a wide spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8.
Primers sequences, genome positions and the size of PCR products are shown in Table 1. The wujeszky chain reaction PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been reported 37915 aujesszky is no standard procedure sjinos so far 2. Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.
This paper describes the development, optimization and performance assessment of a rapid and highly sensitive PCR test for detection of pseudorabies virus. Iowa State University Press; Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR.
Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier. Can J Com Med. A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies. This was possible due to the high doen temperature of the primers pair designed and contributes to the reaction efficiency. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs.
Published online Sep 1. The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. Thus, the optimal concentration of MgCl2 and primers were 1. Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract.