Crystallization of Biological Macromolecules, by Alexander McPherson. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory. Crystals of macromolecules have become keystones of molecular biology, bridging Chapter 3: The Purification and Characterization of Biological Macromolecules ~xray/Books/ Results 1 – 19 of 19 Crystallization of Biological Macromolecules by Alexander McPherson and a great selection of related books, art and collectibles available.

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For the successful X-ray structure determination of macromolecules, it is first necessary to identify, usually by matrix screening, conditions that yield some sort of crystals.

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The improvement process is referred to as optimization, and it entails sequential, incremental changes in the chemical parameters that influence crystallization, such as pH, ionic strength and precipitant concentration, as well as physical parameters such as temperature, sample volume and overall methodology.

This extensively illustrated book provides a biochemical context in which crystal growth can be pursued and instructs readers crystaloization practical aspects of the technology, laying out effective strategies for success.

Crystallization of biological macromolecules

Browse titles from CSHL. Stress Biology of Cyanobacteria. About this book This extensively illustrated book provides a biochemical context bioolgical which crystal growth can be pursued and instructs readers in practical aspects of the technology, laying out effective strategies for success. Guide to the Human Genome.


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Optimization of crystallization conditions for biological macromolecules.

Even when the initial samples are suitable, often marginally, refinement of kf is recommended in order to obtain the highest quality crystals that can be grown. Cold Spring Harbor Laboratory Press.

It also includes the application of some unique procedures and approaches, and the addition of novel components such as detergents, ligands or other small molecules that may enhance nucleation or crystal development. Customer Reviews Review this book. Salamanders in Regeneration Research.

Optimization of crystallization conditions for biological macromolecules.

About this book Contents Related titles. It is therefore generally necessary to improve upon these initial conditions in order to obtain better crystals of sufficient quality for X-ray data collection.

The quality of an X-ray structure determination is directly correlated with the size and the perfection of the crystalline samples; thus, refinement of conditions should always be a primary component of crystal growth. Have a Nice DNA. Purifying and Culturing Neural Cells.

Click to have a closer look. Praise of Chromosome Folly. Initial crystals are mcaromolecules microcrystals or clusters, and often have unfavorable morphologies or yield poor diffraction intensities.

Here, an attempt is made to provide guidance on how optimization might best be applied to crystal-growth problems, and what parameters and factors might most profitably be explored to accelerate and achieve success.

Crystallization of biological macromolecules for X-ray diffraction studies.

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Exceptional customer service Get specialist help and advice. Contents The history and character of macromolecular crystals; principles of macromolecular structure and the applications of x-ray crystallography; the purification and characterization of biological macromolecules; some physical and energetic principles; practical procedures for macromolecular crystallization; important considerations in macromolecular crystallization; strategies and special cmpherson in growing crystals; impurities, defects, and crystal quality; the mechanisms and kinetics of macromolecular crystal growth; crystal growth in unique environments.

Molecules that Changed the World.